Hydrophobization and bioconjugation for enhanced siRNA delivery and targeting
نویسندگان
چکیده
منابع مشابه
Hydrophobization and bioconjugation for enhanced siRNA delivery and targeting.
RNA interference (RNAi) is an evolutionarily conserved process by which double-stranded small interfering RNA (siRNA) induces sequence-specific, post-transcriptional gene silencing. Unlike other mRNA targeting strategies, RNAi takes advantage of the physiological gene silencing machinery. The potential use of siRNA as therapeutic agents has attracted great attention as a novel approach for trea...
متن کاملsiRNA delivery and targeting.
RNA interference (RNAi) is one of the most dramatic findings over the past decade, and the number of publications related to RNAi increased exponentially from 5 in 1998 to more than 2400 in 2008. Generally, RNAi can be achieved by three strategies: chemical synthesized small interfering RNA (siRNA), long double-stranded RNA (dsRNA), and DNA-based (plasmid or viral vector) short hairpin RNA (shR...
متن کاملTargeted delivery of siRNA to hepatocytes and hepatic stellate cells by bioconjugation.
Previously, we successfully conjugated galactosylated poly(ethylene glycol) (Gal-PEG) to oligonucleotides (ODNs) via an acid labile ester linker (Zhu et al., Bioconjugate Chem. 2008, 19, 290-8). In this study, sense strands of siRNA were conjugated to Gal-PEG and mannose 6-phosphate poly(ethylene glycol) (M6P-PEG) for targeted delivery of siRNAs to hepatocytes and hepatic stellate cells (HSCs),...
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Objective(s): Lipid-based nanoparticles (NLP) are PEGylated carriers composed of lipids and encapsulated nucleic acids with a diameter less than 100 nm. The presence of PEG in the NLP formulation improves the particle pharmacokinetic behavior. The purpose of this study was to prepare and characterize NLPs containing MDR1 siRNA and evaluate their cytotoxicity and cellular uptake. MDR1 siRNA coul...
متن کاملMMP-Responsive, Proximity-activated Targeting Polymeric Nanoparticles for siRNA Delivery
SUMMARY A smart polymeric nanoparticle (SPN) with matrix metalloproteinase-7 (MMP-7) dependent proximityactivated targeting (PAT) has been designed and tested for targeted intracellular siRNA delivery to MMP-7 overexpressing tissues (i.e., tumor metastases). With this novel PAT-SPN design, a removable PEG corona mitigates cytotoxicity and nonspecific uptake in normal tissues. Exposure to MMP (i...
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ژورنال
عنوان ژورنال: RNA
سال: 2007
ISSN: 1355-8382
DOI: 10.1261/rna.459807